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To separate and quantify the conjugates GS-TNB and CYS-TNB, a gradient elution method was implemented using eluent A, 7/10/83 (v/v/v) methanol/100 mM K-phosphate buffer, pH 6/water and eluent B, 30/10/60 (v/v/v) methanol/100 mM K-phosphate buffer, pH 6/water

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