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The sections were deparaffinized, hydrated, antigen retrieved, and then probed with primary antibodies against KIM-1, IL-1, TNF-, F4/80, 4-HNE, GPX4, Ferritin, and p53 at the dilution of 1:200, followed by the incubation with the secondary antibodies for 60min on the second day

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By disrupting the interaction between FOXO4 and p53, FOXO4-DRI induces apoptosis (programmed cell death) in senescent cells, reducing their harmful effects

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However, there is a growing interest in that field

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