Carnitine membrane transporter deficiency: a long-term follow up and OCTN2 mutation in the first documented case of primary carnitine deficiency
2017;9(397):eaan0026
To do this, we first confirmed that both cytosolic- and mitochondrial-targeted Apollo-NADP + sensors are expressed in appropriate compartments of RAW264.7 Ms
10.1172/JCI200421625 43
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Blots were treated with primary antibodies, including anti-lysyl oxidase-like 1 (LOXL1) (1:1000, ab313585, Abcam), anti-periostin (Postn) (1:500, sc-398631, Santa-Cruz), anti-interferon stimulated gene 15 (ISG15) (1:1000, 2743, Cell Signaling Technology), anti-superoxide dismutase-3 (Sod3) (1:1000, ab80946, Abcam), anti-peroxiredoxin (Prdx) 4 (1:1000, ab184167, Abcam), anti-aldehyde dehydrogenase 1a1 (ALDH1a1) (1:1000, ab52492, Abcam), anti-Prdx6 (1:1000, ab133348, Abcam), anti-glutathione S-transferase M1 (GSTM1) (1:1000, PA5-79337, Invitrogen), and anti--actin (1:1000, 4970, Cell Signaling Technology), at 4 C overnight, and IRDye 800 CW-conjugated secondary antibodies for 2 h at room temperature, subsequently