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Important considerations include existing medical conditions and current medications

Questions to discuss include How my eating patterns might change Whether reduced intake could affect vitamin and mineral adequacy Whether working with a registered dietitian would be helpful Observational studies of sustained energy restriction and pharmacologically assisted weight loss have shown that inadequate vitamin and mineral intake can occur when nutrition is not actively monitored

Quantitative reverse transcriptase-PCR was performed using the SYBR Green I Kit (Roche, Mannheim, Germany)

PLoS Negl Trop Dis 9 (9), 1

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