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Apoptosis and cell desquamation in repair process of ischemic tubular necrosis

Following centrifugation at 6,250 g , cell lysate was purified by affinity chromatography using Ni 2+ -Nitrilotriacetic acid (NTA) resin (HisPur Ni-NTA Resin, PI88222, Fisher), as previously (Wright et al., 2018)

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The micelles were prepared with varying degrees of modification to achieve an optimal grafting degree of 1530% regarding the lowest critical micelle concentration (CMC)

The data are presented as mean SD from three independent experiments