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Islets were identified under a dissecting microscope and suspended in RPMI 1640 plus 15% FBS for at least 16 hours prior to experimentation

The medication works through sustained exposure over weeks and months

Since most ingested allulose is absorbed and excreted in urine, with only a fraction reaching the colon, the direct microbiome effects may be less significant than initially hypothesized

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Blood glucose levels were monitored at 10, 20, 30, 60, and 90 min post-injection