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Umbrella Labs encourages laboratories to treat these variables as part of the experimental design, recorded in a way that a different operator can replicate
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In contrast to ciprofloxacin-treated cells, we observed typical kanamycin-treated cells to lyse immediately after hypoosmotic shock (Supplementary Movie 10), as would be consistent with a scenario in which kanamycin-induced membrane damage is more widespreadpotentially due to ribosome-independent ionic interactions with the outer membrane 20,26 and results in a lower resistance of the membranes to stretching