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Cellular migration models

Diabet Med 7:304309 Serri O, Beauregard H, Brazeau P, Abribat T, Lambert J, Harris A, Vachon L (1991) Somatostatin analogue, octreotide, reduces increased glomerular filtration rate and kidney size in insulin-dependent diabetes

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Membranes were washed three times with PBST to remove residual primary antibodies and then incubated with horseradish peroxidase-conjugated goat anti-rabbit or goat anti-mouse IgG secondary antibodies at 25 C for 60 min

Immunofluorescence To assess cell area and morphology, and for quantification of p-FAK, Ki67, MITF, YAP, and p-ERK signal intensities, MCs were seeded on the different substrate combinations at a density of 2500 cells/cm, to avoid overcrowding and allow single cell measurements

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