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Protein-induced membrane curvature alters local membrane tension

The Sepharose was removed through centrifugation, and 4 l aliquots of the supernatant were pipetted onto glow-discharged C-flat holey carbon film R 1.2/1.3 400 mesh Quantifoil grids (EM Sciences, Hatfield, PA)

Common Safety Considerations Users should be aware of: Gastrointestinal effects (nausea, vomiting) Appetite suppression leading to low calorie intake Dehydration if fluid intake is insufficient These effects are usually manageable with proper dosing and lifestyle adjustments

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