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The primary inoculum (1 mL) was transferred into 140 mL of fresh LB medium in a 500 mL flask and cultured for 8 h to obtain the secondary inoculum, which was inoculated directly into the bioreactor

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Plateletneutrophil aggregates formed on this activated surface further amplify thrombin generation and fibrin deposition, establishing a feedforward loop of vascular inflammation and thrombosis [50]

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