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We analysed the Ubx splicing activity on target genes identified previously 21 , including Chascon ( Chas ), regulated at both transcriptional and splicing levels, three genes regulated solely at the splicing level, Puratrophin ( Pura ), polyA binding protein ( pAbp ), Dunce ( Dnc ), and Rad, Gem/Kir family member 1 ( Rgk1 ) exhibiting an intermediary profile with Ubx WT influencing differential splicing, but also transcription only at high Ubx expression level (Figure 1I-J, Figure S2A-N)

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