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The crude product was purified by a silica gel column chromatography using hexane and dichloromethane mixture (10:1 v/v) as eluent and obtained as yellow powder in 74% yield

Rochais F, Vilardaga JP, Nikolaev VO et al (2007) Real-time optical recording of beta1-adrenergic receptor activation reveals supersensitivity of the Arg389 variant to carvedilol

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