Finally, samples were washed with PBS and counterstained with 4,6-diamidino-2-phenylindole (DAPI) (Life Technologies)
The MCSCF method revealed that the mixing of orbitalsthose occupied by electrons and those unoccupied [92]leads to a significant transfer of electron densities [93,94,95], and even electrons [96, 97], from biological substrates to vitamin B12 cofactors, as well as to the intermolecular orbitals formed between the cofactors and active substrates [93,94,95], aligning theoretical results with experimental observations [2, 90]
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