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ALS can also be genetically categorized as being either sporadic ALS (SALS) that represents 9095% of all diagnoses and in which there is no known direct genetic contribution to the disease, or familial ALS (FALS) that makes up the remaining 510% of all cases where known genetic mutations such as in SOD1, TDP-43, FUS, and C9ORF72 predominate (Zarei et al., 2015)

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2.6 Western blot analysis Total proteins were lysed with RIPA buffer (Fude, FD009)

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