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After cell lysis by using a high-pressure homogenizer HPL6 (Maximator GmbH, Nordhausen, DE), the crude lysate was centrifuged for 45 min at 23,545 g , 4 C and the supernatant passed through a 0.2 M filter

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Acknowledgements This study was supported by the Plan on Enhancing Scientific Research in GMU, Guangzhou Clinical Characteristic Technology Project, and Postdoctoral Science Foundation of China (2018M643893)

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doi: 10.1371/journal.pone.0025637 43 ClarkR

Genetic considerations related to intracytoplasmic sperm injection (ICSI)