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Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma #IPVH00010), blocked for an hour using 5% milk in TBST, and stained overnight with targeting primary antibodies in 5% milk TBST

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doi: 10.1111/j.1476-5381.2012.02068.x 60 DoveLSLemboARandallCWFogelRAndraeDDavenportJMet al