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Following purification, the protein was diluted to 0.08 mg/mL in refolding buffer at pH 8.5 (10.56 mM NaCl, 55 mM Tris-HCl, 2.2 mM CaCl2, 2.2 mM MgCl2, 0.055% PEG4000, 0.55 M L-arginine, 0.1 mM oxidized glutathione, and 1 mM reduced glutathione) and incubated overnight at 4C with shaking