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Vitamin B12 pairs well with most supplements and is often taken alongside other B vitamins, iron, vitamin D, and multivitamins as part of a comprehensive wellness routine

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Then, UPLC-grade water DDW:MetOH (3:1, v/v) solution (0.5 mL) containing internal standards: 13 C- and 15 N-labeled amino acids standard mix (Sigma-Aldrich) was added to the tubes, followed by centrifugation