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It is worth noting that Eikan et al

The pressure can damage the peptide structure

The HA tag is cleaved and loses immunoreactivity during apoptosis

[PubMed: 20020370] 165

Moreover, in a validation study to confirm the reproducibility of our findings, the glutathione level was reduced in NTG and POAG patients compared with a cataract control group

Advanced imaging techniques, including confocal imaging, and high-throughput data acquisition with microplate readers, further support the validation process by enabling precise, real-time monitoring of roGFP fluorescence in different cell types and cellular compartments