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This can be prevented by derivatizing GSH with the alkylating agent N -ethylmaleimide (NEM) to form GS-NEM before acid deproteination, followed by back-extraction of excess NEM from the deproteinized samples with dichloromethane

Biochim Biophys Acta 1830:330416 Markovic J, Mora NJ, Broseta AM, Gimeno A, de-la Concepcin N, Vina J, Pallardo FV (2009) The depletion of nuclear glutathione impairs cell proliferation in 3 t3 fibroblasts

doi: 10.1016/j.freeradbiomed.2015.12.024

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