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149 Furthermore, certain drugs like amitriptyline, commonly prescribed for depression, have been found to bind reversibly to the G-sites of GSTP1 and GSTA1, leading to a significant reduction in their activity

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To study the permeability of nanoparticles in vitro BBB models, which conform to the 3R principles with the minimalization the number of animal experiments, are widely used either in static [34] or in dynamic conditions by employing lab-on-a-chip systems [35]