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A portion of brain tissue (100 mg) was taken in a 900-microliter volume of PBS (1:9 ratio) in a glass homogenizer on ice and homogenized at 2,000 rpm for 5 min

doi: 10.1093/humupd/dmaa029 94 GomezJVanHiseKStachenfeldNChanJLMerzNBShufeltC

This is especially relevant for people doing interval training, cardio-intensive workouts or strength endurance training

(2002) Gut hormone PYY(3-36) physiologically inhibits food intake