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(2023) estimated that over 1,000 participants per group would be required in ME/CFS studies to account for misclassification, a scale beyond current studies (Malato et al., 2023)

For certain conditions and populations, GHK-Cu is not just inadvisable

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One such example involves utilizing a smaller Cas9 ortholog from Staphylococcus aureus (SaCas) (3.15 kb), which is ~1 kb shorter than the SpCas9, so that the CRISPR-Cas9 construct and gRNA can fit within a single AAV vector [159, 160]

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