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IGF1R activation assay 293FT cells (R70007, Invitrogen) were cultured in high-glucose DMEM supplemented with 10% (v/v) FBS, 2 mM L-glutamine, and 1% penicillin/streptomycin

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Furthermore, -HB exerts direct regulatory influences on K+ channels and neuronal vesicular glutamate transporters (66) and it inhibits inflammation mediated by the NOD-like receptor family pyrin domain-containing 3 (NLRP3) (67)