B12 is found almost exclusively in animal products meat, fish, dairy, eggs
However, translating findings from basic research into clinical applications still poses challenges
OsDHAR was purified by Ni-NTA affinity chromatography [5 mL HisTrap FF column (GE Healthcare, Little Chalfont, UK) on an AKTA FPLC system] and eluted with a step gradient of 250 mM imidazole
V.BaldychevaA.et al (2023)
doi: 10.2967/jnumed.120.261594 76 WangBPeiJXuSLiuJYuJ
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