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The recombinant protein was eluted with 10 mL of elution buffer (equilibration buffer containing 400 mM imidazole)

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During the distribution phase, a second peak was observed at 120 min, followed by a terminal phase with a half-life of 2.2 h (Liu et al., 2013)

About 85% of lung cancers are associated with smoking, however, lung cancer occurs also among non-smokers and represents the seventh most common cause of cancer mortality which indicates that other factors such as genetic predisposition, diet, silica, asbestos, high energy radiation, concentration of radon and other factors play a role