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Peptides are naturally occurring amino acid chains that help regulate important processes like metabolism, tissue repair, energy, and cellular signaling

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After incubation, the membrane was washed three times with TBST (Servicebio, G0004) and then incubated with an HRP-conjugated secondary antibody (Jackson ImmunoResearch, 111-005-003, 1:100,000 dilution) for 2 h at room temperature

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