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We analyzed the relative signal densities by ImageJ software, and -actin density was used as the internal control
In DMSO-treated control, only few endothelial cells migrated from the upper to the lower chamber through the membrane after 12-hour incubation, when the lower chamber contained culture medium supplemented with 10% FBS as a chemoattractant
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[23] Cyanocobalamin is usually converted to hydroxocobalamin in the serum, whereas hydroxocobalamin is converted to either methylcobalamin or adenosylcobalamin