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Upon DEM treatment, we observe increased Nrf2-GFP levels, however Keap1-GFP is hardly induced due to the siKEAP1 treatment (Fig 3A,B)

Thats why you shouldnt assume that you wont develop stretch marks just because you arent overweight

On the other hand, the reason why Fas/FasL signaling, caspase-8, and caspase-3 are retained in mature erythrocytes could be to provide the interplay between eryptosis and necroptosis of erythrocytes

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Are there step therapy requirements (meaning I need to try other medications first)

The physiological changes that produced obesity, including altered appetite regulation, impaired satiety signaling, and metabolic adaptations, do not resolve permanently with weight loss alone