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GPX4 activity is dependent on Glutathione (GSH) which is synthesized using cystine imported via the cystine-glutamate antiporter, System Xc

Its structure incorporates several non-natural amino acid modifications that enhance receptor binding affinity and metabolic stability compared to endogenous ghrelin

R-CQDs, red fluorescent CQDs

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Antibodies used were rabbit anti-ACSL4 (1:1000) (ThermoFisher, #PA527137, RRID: AB_2544613), anti-GAPDH (1:1000) (Cell Signaling, #5174, RRID: AB_10622025), and anti-GPX4 (1:1000) (ThermoFisher, #MA532827, RRID: AB_2810103)

8023, revised 1978)