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Spectrophotometric study of Zn(II) dissociation with PAR All experiments with PAR were conducted using a JASCO V-650 spectrophotometer with a Peltier module ETCS-761 and a buffer of 50 mM HEPES-Na + , 100 mM NaCl, pH 6.5, 7.4, or 8.5 in a 1 cm quartz cuvette

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